Optimization of CTLA-4 and PD-1 proteins in EMT6 Mouse Mammary Cancer Cells by Western Blot

Targeting the activation of immune checkpoints is recognized as an effective strategy for triggering anti-tumour responses in cancer cells. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1) were identified potential crucial targets treatment. Overexpression CTLA-4 PD-1 proteins primary tumour human lines well documented. In contrary, lack data was available using animal lines. The presence study aims to optimize expression EMT6 mouse mammary cells Western blot, provide basic understanding their association with breast progression. Proteins extracted from parental adjusted 30ng gel electrophoresis. Afterwards, transferred a nitrocellulose membrane blotting. then subjected chemiluminescent band detection. Results obtained beta-actin housekeeping gene show that both (32 kDa) (34 expressed by 1:1000 dilution each antibody lysate relative (4.0 ± 0.26) higher compared (1.2 1.8). As conclusion, indeed this outcome platform extensive vivo research on link model.